弊社取扱製品が活用された、COVID-19・ウイルス、感染症関連研究をご紹介致します。(2022/8/5更新)
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ゲノム・遺伝子｜
タンパク質・細胞解析｜
in vivoイメージング｜
実験動物 in vivo生体計測｜
新型コロナウイルス研究・PCR検査ソリューション 
＜ゲノム・遺伝子＞
Francesca Di Giallonardo et al.,
Genomic Epidemiology of the FirstWave of SARS-CoV-2 in Italy
Viruses 2020, 12, 1438; doi:10.3390/v12121438
https://www.mdpi.com/1999-4915/12/12/1438
※Arbor Biosciences社 myBaitsが使用されています。
Hawman et al.,
SARS-CoV2 variant-specific replicating RNA vaccines protect from disease following challenge with heterologous variants of concern
eLife 2022;0:e75537. DOI:
https://doi.org/10.7554/eLife.75537
※Telesis Bio (旧 Codex DNA)社 BioXp使用。
Telesis Bio (旧 Codex DNA)社 Application Note(日本語訳版)
SARS-CoV-2が引き起こすCOVID-19と戦うためにBioXp™3200システムの能⼒を利⽤する 
※旧モデルBioXp3200を活用した、SARS-CoV-2の全ゲノムのオンデマンド⽣成について説明されています。
米国CBS6チャンネルでのNEWS：2020/12/31
GENETWORXでの1日50,000検体のoKtopureによるDNA抽出とIntelliQubeによる試験が紹介されました。
https://www.youtube.com/watch?v=LBlBV4QKbmk 
Youtubeチャンネル：WTVR CBS 6 (3分16秒、字幕・自動翻訳(日本語)利用：可)
Charlotte B. Acharya et al.,
No Significant Difference in Viral Load Between Vaccinated and Unvaccinated, Asymptomatic and Symptomatic Groups When Infected with SARS-CoV-2 Delta Variant
medRxiv,
https://doi.org/10.1101/2021.09.28.21264262
※LGC BioSearch社 IntelliQubeが使用されています。
Charlotte B. Acharya et al.,
No Significant Difference in Viral Load Between Vaccinated and Unvaccinated, Asymptomatic and Symptomatic Groups Infected with SARS-CoV-2 Delta Variant
medRxiv, doi. :10.1101/2021.09.28.21264262
https://www.medrxiv.org/content/10.1101/2021.09.28.21264262v1
※LGC BioSearch社 IntelliQubeが使用されています。
＜タンパク質・細胞解析＞
Justin D. Walter et al.,
Highly potent bispecific sybodies neutralize SARS-CoV-2
bioRxiv, 2020 DOI : 10.1101/2020.11.10.376822
https://www.biorxiv.org/content/10.1101/2020.11.10.376822v1
※Creoptix社WAVEシステムが活用されています。
Lara Gibellini et al.,
Altered bioenergetics and mitochondrial dysfunction of monocytes in patients with COVID‐19 pneumonia
EMBO Mol Med (2020)e13001 | doi. :10.15252/emmm.202013001
https://www.embopress.org/doi/full/10.15252/emmm.202013001
※Agilent社 Seahorse XF フラックスアナライザーが使用されています。
Saima Ajaz et al.,
Mitochondrial metabolic manipulation by SARS-CoV-2 in peripheral blood mononuclear cells of COVID-19 patients
Cell Physiology | Call for Papers: Virus-Host Cell Interactions and the Viral Life Cycle: Basic Science to Therapeutics | doi.:10.1152/ajpcell.00426.2020
https://journals.physiology.org/doi/abs/10.1152/ajpcell.00426.2020
※Agilent社 Seahorse フラックスアナライザー XFpが使用されています。
Agilent社 Webinar (2019/4/4実施分、オンデマンド視聴) ※対象疾患：結核
Exploring how Mycobacterium tuberculosis reprograms host bioenergetic metabolism
https://seahorseinfo.agilent.com/acton/fs/blocks/showLandingPage/a/10967/p/p-01c3/t/page/fm/1
演者：Bridgette M Cumming, PhD
Research Associate, Africa Health Research Institute (AHRI) Durban, South Africa
※簡単なご登録後に視聴いただけます。
Agilent社 Application Brief (日本語訳版)
※対象：ノロウイルス
Agilent Seahorse XF テクノロジーはノロウイルス研究に強力な機能的計測を提供する
※ノロウイルス感染細胞では解糖によるATP産生の比率が増えることが示されています。
※対象：インフルエンザ
CD8+ T細胞の代謝の制御はインフルエンザウイルス感染から保護する
※対象：RSウイルス
宿主細胞のミトコンドリア機能に対するRSウイルス感染の影響の研究
▲ ページの先頭へ戻る
＜in vivoイメージング＞【動物種｜疾患名｜使用モダリティ｜装置モデル】
【マウス｜SARS-CoV-2｜PET/CT｜NanoScanPET/CT】
Yang, Y., Wu, Y., Meng, X. et al.
SARS-CoV-2 membrane protein causes the mitochondrial apoptosis and pulmonary edema via targeting BOK
Cell Death & Differentiation, Published: 12 January 2022
https://www.nature.com/articles/s41418-022-00928-x
【ハムスター｜SARS-CoV-2｜PET/CT｜NanoScanPET/CT+ Vivoquant2020】
Camilo A. Ruiz-Bedoya et al.,
124I‑Iodo‑DPA‑713 Positron Emission Tomography in a Hamster Model of SARS‑CoV‑2 Infection
Molecular Imaging and Biology (2021),
https://doi.org/10.1007/s11307-021-01638-5
※画像解析にVivoQuant (Invicro)を使用。
【カニクイザル｜H5N1 インフルエンザ｜MultiScan LFER 150 PET-CT + VivoQuant】
Petra Mooij et al.,
Aerosolized Exposure to H5N1 Influenza Virus Causes Less Severe Disease Than Infection via Combined Intrabronchial, Oral, and Nasal Inoculation in Cynomolgus Macaques
Viruses 2021, 13, 345
https://doi.org/10.3390/v13020345
【マウス｜SARS-CoV-2｜SPECT/CT｜NanoSPECT/CT(旧モデル) + Vivoquant】
Pyrć, K., Milewska, A., Duran, E.B. et al.,
SARS-CoV-2 inhibition using a mucoadhesive, amphiphilic chitosan that may serve as an anti-viral nasal spray
Sci Rep 11, 20012 (2021),
https://www.nature.com/articles/s41598-021-99404-8
※画像解析にVivoQuant (Invicro)を使用。
【マウス｜SARS-CoV-2｜SPECT/CT｜NanoSPECT/CT(旧モデル) + Vivoquant】
Krzysztof Pyrć et al.,
SARS-CoV-2 inhibition in human airway epithelial cells using a mucoadhesive, amphiphilic chitosan that may serve as an anti-viral nasal spray
bioRxiv, December 10,2020,
https://doi.org/10.1101/2020.12.10.413609
※哺乳類の鼻上皮細胞へのウイルスの侵入を防ぐための点鼻薬候補薬剤の評価をマウスで実施
※画像解析にVivoQuant (Invicro)を使用。
【ウサギ｜結核 ｜ PET/CT｜ nanoScan PET/CT】
Alvaro A. Ordonez et al.,
Dynamic imaging in patients with tuberculosis reveals heterogeneous drug exposures in pulmonary lesions
Nature Medicine volume 26, pages529–534(2020)
https://doi.org/10.1038/s41591-020-0770-2
※コロナウイルス関連の報告でよく引用されている論文です。
【マカクザル｜SARS-CoV-2｜LFER150 PET/CT + Vivoquant】
Ingrid H.C.H.M. Philippens et al.,
SARS-CoV-2 causes brain inflammation and induces Lewy body formation in macaques
bioRxiv, doi.: 10.1101/2021.02.23.432474
https://www.biorxiv.org/content/10.1101/2021.02.23.432474v2
【サル｜SARS-CoV-2 ｜ PET/CT｜ LFER150 PET/CT + Vivoquant】
Dhiraj Kumar Singh et al.,
SARS-CoV-2 infection leads to acute infection with dynamic cellular and inflammatory flux in the lung that varies across nonhuman primate species
bioRxiv, doi. :10.1101/2020.06.05.136481
https://www.biorxiv.org/content/10.1101/2020.06.05.136481v1
CT Imaging and quantitative analysis of lung pathology
【アフリカミドリザル｜SARS-CoV-2 ｜ PET/CT｜ LFER150 PET/CT + 体温・呼吸｜テレメトリー／ヘッドアウトプレチスモグラグ 】
Amy L. Hartman et al.,
SARS-CoV-2 infection of African green monkeys results in mild respiratory disease discernible by PET/CT imaging and shedding of infectious virus from both respiratory and gastrointestinal tracts
PLOS PATHOGENS | Published: September 18, 2020 |
10.1371/journal.ppat.1008903
【サル｜SARS-CoV-2 ｜ PET/CT｜ LFER150 PET/CT + Vivoquant】
Dhiraj Kumar Singh et al.,
Responses to acute infection with SARS-CoV-2 in the lungs of rhesus macaques, baboons and marmosets
Nature Microbiology volume 6, pages73–86(2021)
https://www.nature.com/articles/s41564-020-00841-4
CT Imaging and quantitative analysis of lung pathology
【インド起源アカゲザル・南アジア起源カニクイザル｜SARS-CoV-2｜LFER150 PET/CT + Vivoquant】
Kinga P. Böszörményi et al.,
The Post-Acute Phase of SARS-CoV-2 Infection in Two Macaque Species Is Associated with Signs of Ongoing Virus Replication and Pathology in Pulmonary and Extrapulmonary Tissues
Viruses 2021, 13, 1673, doi.: 10.3390/v13081673
https://www.mdpi.com/1999-4915/13/8/1673
【光イメージング】Webinarオンデマンド
Spectral Instruments Imaging社 Webinar (2020/4/28公開、31分｜英語)
Optical Imaging Webinar: Viral Pathogenesis & SARS-CoV2 Drug Discovery
https://youtu.be/Jio-QuEG5nM
【超音波】Webinarオンデマンド
FUJIFILM Visualsonics社 Webinar (2020/5/11実施分、47分(プレゼン約30分+質疑応答約15分)｜英語)
From Bedside to Bench: Application of Lung Ultrasound in Small Animals
演者：Jana Grune, PhD and Niklas Hegemann, MSc from Charité – Universitätsmedizin Berlin
https://youtu.be/r_iTIr8MeZI
▲ ページの先頭へ戻る
＜実験動物 in vivo生体計測＞【動物種｜疾患名｜計測項目｜使用システム】
【ハムスター｜SARS-CoV-2｜Whole Body Plethysmography + 酸素飽和度｜MouseOxPLUS】
Suzuki, R., Yamasoba, D., Kimura, I. et al.
Attenuated fusogenicity and pathogenicity of SARS-CoV-2 Omicron variant
Nature (2022), doi. :10.1038/s41586-022-04462-1
https://www.nature.com/articles/s41586-022-04462-1 Full Text Link (PDF)
Research Square (2022), BIOLOGICAL SCIENCES - ARTICLE, doi.:10.21203/rs.3.rs-1207670/v1
https://www.researchsquare.com/article/rs-1207670/v1 Full Text Link (PDF)
【マウス｜SARS-CoV-2 ｜Whole Body Plethysmography】
Becker J, et al
Ex vivo and in vivo suppression of SARS-CoV-2 with combinatorial AAV-RNAi expression vectors
Molecular Therapy(2022), 12 January 2022, ji2000751; doi. :10.1016/j.ymthe.2022.01.024
https://www.sciencedirect.com/science/article/pii/S1525001622000247 Full Text Link (PDF)
【マウス｜Influenza A ｜Whole Body Plethysmography】
SM Hartwig, AM Miller, SM Varga, et al
Respiratory Syncytial Virus Provides Protection against a Subsequent Influenza A Virus Infection
J Immunol January 12, 2022, ji2000751; doi. :10.4049/jimmunol.2000751
https://www.jimmunol.org/content/early/2022/01/10/jimmunol.2000751.abstract
【ハムスター｜SARS-CoV-2｜Whole Body Plethysmography】
Saito, A., Irie, T., Suzuki, R. et al
Enhanced fusogenicity and pathogenicity of SARS-CoV-2 Delta P681R mutation.
Nature (2021), Accelerated Article Preview, doi.:10.1038/s41586-021-04266-9
https://doi.org/10.1038/s41586-021-04266-9 Full Text Link (PDF)
【マウス・ハムスター｜SARS-CoV-2｜Elastance, Resistance, and dynamic or static compliance｜Resistance & Compliance】
Yamaguchi, T., Hoshizaki, M., Minato, T. et al.,
ACE2-like carboxypeptidase B38-CAP protects from SARS-CoV-2-induced lung injury
Nat Commun 12, 6791 (2021)
https://doi.org/10.1038/s41467-021-27097-8 Full Text Link (PDF)
【マウス｜SARS-CoV-2｜PenH他｜Whole Body Plethysmography】
Sarah R. Leist et al.,
A Mouse-Adapted SARS-CoV-2 Induces Acute Lung Injury and Mortality in Standard Laboratory Mice
2020, Cell 183, 1070–1085, November 12, 2020
https://doi.org/10.1016/j.cell.2020.09.050 Full Text Link (PDF)
【マウス｜SARS-CoV-2｜Whole Body Plethysmography】
Antonella Conforti, et al.,
COVID-eVax, an electroporated DNA vaccine candidate encoding the SARS-CoV-2 RBD, elicits protective responses in animal models
Mol Ther. 2021 Sep 20, doi: 10.1016/j.ymthe.2021.09.011 [Epub ahead of print]
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483992/ Full Text Link (PDF)
【マウス｜SARS-CoV-2｜PenH他｜Whole Body Plethysmography】
Kenneth H. Dinnon III, et al.,
A mouse-adapted model of SARS-CoV-2 to test COVID-19 countermeasures
Nature volume 586, pages560–566(2020), Published: 27 August 2020
https://www.nature.com/articles/s41586-020-2708-8 Full Text Link (PDF)
【マウス｜COVID-19, SARS-CoV-2｜PenH｜Whole Body Plethysmography】
Pruijssers AJ et al.,
Remdesivir potently inhibits SARS-CoV-2 in human lung cells and chimeric SARS-CoV expressing the SARS-CoV-2 RNA polymerase in mice
https://europepmc.org/article/ppr/ppr155890 Full Text Link (PDF)
bioRxiv, 27 Apr 2020, DOI: 10.1101/2020.04.27.064279 PPR: PPR155890
Figure 5. Remdesivir (RDV) is active against the SARS-CoV-2 RdRp in vivo.
G. Pulmonary function by whole-body plethysmography.
The PenH metric shown is a surrogate marker of pulmonary obstruction.
P < 0.0001 as determined by two-way ANOVA with Sidek’s multiple comparison test.
【マウス｜MERS-CoV｜PenH｜Whole Body Plethysmography】
Timothy P. Sheahan et al.,
Comparative therapeutic efficacy of remdesivir and combination lopinavir, ritonavir, and interferon beta against MERS-CoV
Nature Communications volume 11, Article number: 222 (2020) , doi: 10.1038/s41467-019-13940-6
https://www.nature.com/articles/s41467-019-13940-6 Full Text Link (PDF)
Fig. 4: Prophylactic LPV/RTV + IFNb does not improve disease outcomes.
【アフリカミドリザル｜MERS-CoV｜呼吸数・体温｜Whole Body Plethysmography(大動物用)／テレメトリー】
Allison Totura et al.,
Small Particle Aerosol Exposure of African Green Monkeys to MERS-CoV as a Model for Highly Pathogenic Coronavirus Infection
Centers for Disease Control and Prevention, EMERGING INFECTIONUS DISEASES, ISSN: 1080-6059,
Volume 26, Number 12 - December 2020
https://wwwnc.cdc.gov/eid/article/26/12/20-1664_article
Disclaimer: Early release articles are not considered as final versions.
Any changes will be reflected in the online version in the month the article is officially released.
【アフリカミドリザル｜SARS-CoV-2 ｜ PET/CT｜ LFER150 PET/CT + 体温・呼吸｜テレメトリー／ヘッドアウトプレチスモグラグ 】
Amy L. Hartman et al.,
SARS-CoV-2 infection of African green monkeys results in mild respiratory disease discernible by PET/CT imaging and shedding of infectious virus from both respiratory and gastrointestinal tracts
PLOS PATHOGENS | Published: September 18, 2020 |
10.1371/journal.ppat.1008903
【インド起源アカゲザル・南アジア起源カニクイザル｜SARS-CoV-2｜体温・活動量｜PhysioTel Digital(大動物用)／テレメトリー】
Kinga P. Böszörményi et al.,
The Post-Acute Phase of SARS-CoV-2 Infection in Two Macaque Species Is Associated with Signs of Ongoing Virus Replication and Pathology in Pulmonary and Extrapulmonary Tissues
Viruses 2021, 13, 1673, doi.: 10.3390/v13081673
https://www.mdpi.com/1999-4915/13/8/1673
Data Sciencies Internaltion社 Webinar (2020/5/28実施分)
Infectious Disease Research: A Holistic Approach to Physiologic Data Collection and Model Development
感染症研究：生理学的データ収集とモデル開発への総体的アプローチ
演者1：Anna Honko, Ph.D.,
Research Associate Professor, Microbiology, Investigator, National Emerging Infectious Disease Laboratories (NEIDL)
Boston University School of Medicine
演者2：Doug Reed, Ph.D.,
Associate Professor of Immunology, Center for Vaccine Research
University of Pittsburgh School of Medicine
オンデマンド視聴はこちらから。
https://register.gotowebinar.com/register/6762502693406155792
【マウス｜SARS-CoV-2｜酸素飽和度｜MouseOxPLUS】
Jose M. Adrover et al.,
Disulfiram inhibits neutrophil extracellular trap formation protecting rodents from acute lung injury and SARS-CoV-2 infection
JCI Insight. 2022.
https://doi.org/10.1172/jci.insight.157342
【マウス｜SARS-CoV-2｜酸素飽和度｜MouseOx】
Forrest Jessop et al.,
Impairing RAGE signaling promotes survival and limits disease pathogenesis following SARS-CoV-2 infection in mice
JCI Insight, 2022;7(2):e155896, doi. :10.1172/jci.insight.155896
https://insight.jci.org/articles/view/155896
【マウス｜SARS-CoV-2｜酸素飽和度｜MouseOxPLUS】
Bi, Z., Hong, W., Que, H. et al.,
Inactivated SARS-CoV-2 induces acute respiratory distress syndrome in human ACE2-transgenic mice
Signal Transduction and Targeted Therapy volume 6, Article number: 439 (2021),
doi. :10.1038/s41392-021-00851-6
https://www.nature.com/articles/s41392-021-00851-6
【マウス｜Influenza｜酸素飽和度｜MouseOxPLUS】
Jeffrey E. Gotts et al.,
Delayed angiopoietin-2 blockade reduces influenza-induced lung injury and improves survival in mice
Physiological Reports. 2021;9:e15081., First published: 09 November 2021
https://doi.org/10.14814/phy2.15081
【マウス｜Influenza｜酸素飽和度｜MouseOxPLUS】
Natalia V. Bogatcheva et al.,
Concentrated Secretome of Adipose Stromal Cells Limits Influenza A Virus-Induced Lung Injury in Mice
Cells 2021, 10(4), 720; doi.: 10.3390/cells10040720
https://www.mdpi.com/2073-4409/10/4/720
【マウス｜SARS-CoV-2｜酸素飽和度｜MouseOxPLUS】
Seungjin Ryu et al.,
Ketogenic diet restrains aging-induced exacerbation of coronavirus infection in mice
eLife 2021;10:e66522 DOI: 10.7554/eLife.66522
https://elifesciences.org/articles/66522
【マウス｜急性肺損傷｜酸素飽和度｜MouseOxPLUS】
Athanassios Fragoulis et al.,
Reference Gene Selection for Gene Expression Analyses in Mouse Models of Acute Lung Injury
Int. J. Mol. Sci. 2021, 22(15), 7853; doi.: 10.3390/ijms22157853
https://www.mdpi.com/1422-0067/22/15/7853/htm
【マウス｜SARS-CoV-2｜酸素飽和度｜MouseOxPLUS】
Hua Qing et al.,
Type I Interferon Limits Viral Dissemination-Driven Clinical Heterogeneity in a Native Murine Betacoronavirus Model of COVID-19
bioRxiv, 2020, doi.: 10.1101/2020.09.11.294231
https://www.biorxiv.org/content/10.1101/2020.09.11.294231v1.abstract
【マウス｜SARS-CoV-2｜酸素飽和度｜MouseOxPLUS】
Seungjin Ryu et al.,
Ketogenesis restrains aging-induced exacerbation of COVID in a mouse model
bioRxiv, 2020, doi.: 10.1101/2020.09.11.294363
https://www.biorxiv.org/content/10.1101/2020.09.11.294363v1.abstract
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使用された装置のご紹介
ゲノム・遺伝子関連製品
タンパク質・細胞解析装置
in vivoイメージング装置
実験動物 in vivo生体情報計測システム
製造元
＜ゲノム・遺伝子関連製品＞
＜タンパク質・細胞解析装置＞
＜in vivoイメージング装置＞
＜実験動物 in vivo生体計測システム＞
